TABLE 1.
Peptides of the 38-kDa mycobacterial lipoglycoprotein and corresponding lipopeptides used
| Designation | Position (length in aa) | Amino acid sequencea | MHC-I restriction |
|---|---|---|---|
| Controls | |||
| Lymphocytic choriomeningitis virus | 118–126 (10) | RPQASGVYM | H-2Ld |
| Influenza virus nucleoprotein | 366–374 (9) | ASNENMETM | H-2Db |
| Peptidesb | |||
| P1 | 97–108 (12) | AGTVNIGASDAY | H-2Db |
| P2 | 142–150 (9) | HLKLNGKVL | H-2Db |
| P3 | 166–175 (10) | IAALNPGVNL | H-2Db |
| P4 | 309–318 (10) | YPIINYEYAI | H-2Db |
| P5 | 340–349 (10) | ITDGNKASFL | H-2Db |
| Lipopeptides | |||
| LP1 | 97–108 (12) | PA-CSS AGTVNIGASDAY | H-2Db |
| LP3 | 166–175 (10) | PA-CSS-IAALNPGVNL | H-2Db |
| LP4 | 309–318 (10) | PA-CSS-YPIINYEYAI | H-2Db |
a
Predicted anchor residues are underlined. PA, palmitic acid.
b
Based on the amino acid sequence of the 38-kDa lipoglycoprotein published by Vordermeier et al. (34).