TABLE 2.
CTL responses to peptide and lipopeptide P3
| Combination | % Cytotoxicity at T/E ratio givena
|
|||
|---|---|---|---|---|
| EX-38b
|
EL-4c
|
|||
| 1:40 | 1:20 | 1:40 | 1:20 | |
| Lipopeptide-IFA | 28 ± 14 | 34 ± 10 | 3 ± 5 | 1 ± 2 |
| Peptide-IFA | 10 ± 6 | 3 ± 3 | 0 ± 1 | 0 ± 0 |
a
CTL responses (percentage of cytotoxicity) measured after an in vitro restimulation of the in vivo-primed spleen cells with mitomycin-treated EX-38 cells. Results are presented as means ± standard deviations of three experiments. Statistical differences were found between lipopeptide and peptide at a T/E ratio of 1:20 with target cell EX-38 (P < 0.005), between target cells EX-38 and EL-4 for lipopeptide at T/E ratios of 1:40 and 1:20 (P < 0.0025 and P < 0.025, respectively), and for peptide at a T/E ratio of 1:40 (P < 0.025).
b
Target cell line transfected with the gene encoding the 38-kDa lipoglycoprotein.
c
Target cell line used as a negative control.