FIG. 3.

BALB/C mice develop humoral immunity to AhpC during the course of infection with S. typhimurium. Western blot analysis was performed on pooled sera from groups of mice prior to infection with MPG479 (B) or at 14 (C) or 28 (D) days postinfection. (A) Standard SDS-PAGE (12.5% [vol/vol] acrylamide) loaded with a whole-cell lysate from SL1344 (lane 1) or with 2 μg of purified His-tagged AhpC (lane 2). The order of sample loading is maintained throughout the panels. Bound antibody was detected with a rabbit anti-mouse antibody conjugated to alkaline phosphatase as previously described (38). The migration positions of prestained molecular mass markers (in kilodaltons) are indicated (M).