TABLE 1.
Bacterial strains and plasmids used in this studya
| Bacterial strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| BL21(DE3) | F−omp+ rB− mB− (DE3 is a λ lysogen bearing lacI, lacUV5, and gene 1) pLysS | 57 |
| DH5α | F− φ80dlac ΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK−) supE44 thi-1 gyrA96 relA1 λ− | 28 |
| S. typhimurium | ||
| SL1344 | his; virulent mouse pathogen | 31 |
| SL1346 | hisG46 aroA554::Tn10 | 31 |
| MPG203 | ahpC::Mudlux derivative of SL1344 | 25 |
| MPG473 | ahp::cml derivative of SL1344 | This study |
| MPG484 | oxyR::kan derivative of SL1344 | This study |
| MPG479 | aroA554::Tn10 derivative of SL1344 | This study |
| MPG481 | BL21(DE3)(pLysS) containing pPDT14 | This study |
| Plasmids | ||
| pBR322 | Apr Tcr | 4 |
| pBR325 | Apr Tcr Cmr | 5 |
| pGEM-T | TA cloning vector; Apr | Promega |
| pUC4-K | Apr Kmr | Pharmacia |
| pET-19b | T7 expression vector; Apr | Novagen |
| pPDT5 | pBR322 ahpCFSt; Apr | This study |
| pPDT6 | pPDT5 ahp::cml Apr Cmr | This study |
| pPDT7 | pGEM-T oxyRSt Apr | This study |
| pPDT8 | pPDT7 oxyR::kan Apr Kmr | This study |
| pPDT14 | pET-19b ahpCSt Apr | This study |
a
Apr, ampicillin resistance; Tcr, tetracycline resistance; Cmr, chloramphenicol resistance; Kmr, kanamycin resistance; St, S. typhimurium.