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. 2024 Feb 1;19(2):e0295312. doi: 10.1371/journal.pone.0295312

Fig 5. Differences in the proteomic outcomes of IFN-γ stimulation of AM vs MN are not associated with major functional proteins of the IFN-γ proximal signaling pathway.

Fig 5

a) IFNGR1/GAPDH relative protein abundance in MN and AM with and without overnight (o/n) IFN-γ stimulation shows robust receptor levels that were not associated with treatment. b) STAT1/GAPDH relative protein abundance over time in AM and MN in the presence and absence of IFN-γ show abundant constitutive STAT1 levels in AM and up-regulation of STAT1 in MN following overnight treatment. c) P-STAT1/GAPDH protein abundance in AM and MN show phosphorylation of STAT1 (Tyr701) in response to IFN-γ and upregulation of in MN after overnight treatment. d) SOCS1/GAPDH protein abundance time course in the presence and absence of IFN-γ showed low levels that were not associated with treatment. Of note, due to limited cell numbers obtainable from human BAL procedures, Fig 5b-5d were performed with a single blot each for AM and MN studies, accounting for the identical GAPDH bands throughout. Blots were stripped before re-use with alternate probes. Band intensities were normalized to GAPDH levels (N = 4). Representative western blots are shown above the plots. Bar plots are mean +/- 95% CI. Symbol shapes (●■▲*) represent individual donors.