Fig 2. M. oryzae BIP1 locus.

The BIP1 gene is composed of three introns (black lines) and four exons with a 5’-UTR of 234 bp, a 3’-UTR of 1217 bp (both gray boxes) and a CDS of 798 bp (black arrows). 5’-RACE identified transcription starts at positions –53 bp and– 234 bp from the ATG (grey arrows). In the M763 insertion mutant, plasmid pAN7.1 (white triangle) was inserted in the third BIP1 intron, downstream of the exon encoding the bZIP domain. Restriction sites used for cloning are displayed. The BIP1 protein (266 aa) has a bZIP domain located near the N-terminal end (white box).