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. 2024 Jan 22;20(1):e1011945. doi: 10.1371/journal.ppat.1011945

Fig 7. BIP1 binds to a GCN4-like TGACTC motif.

Fig 7

(A) Radioactively labeled oligonucleotide probes centered on the TGACTC motifs of promoters from MGG_08381/MGG08380 (ORF3/RAP2), MGG_02201 (Peptidase), MGG_03584 (PTH11-like), MGG_06535 (PTH11-like) genes were incubated in the presence of binding buffer (FP, free probe), BIP1 rabbit reticulocyte lysate (BIP1), BIP1 lysate with unlabeled probe (1:2 or 1:10 molar ratios) or rabbit reticulocyte lysate lacking the BIP1 expression construct (Lysate) before non-denaturing PAGE. For MGG_08381/MGG08380 (ORF3/RAP2), oligo probes carrying a mutation in the first or both GCN4 motif were tested in addition to the wild-type oligonucleotide. The mutant oligonucleotide probes pORF3-mut1 and pORF3-mut2 are shown with mutated bases in blue. (B) The oligonucleotides bound by BIP1 in EMSA were aligned on the TGACTC core sequence and submitted to WebLogo to define the BIP1-binding consensus motif.