Table 1. Genes down-regulated in Δbip1 appressoria differentiated on Teflon.
The expression ratios are log2 transformed. * Comparison of appressoria and in vitro grown mycelium. RNA seq comparing appressoria and germinating conidia from Osés-Ruiz et al., 2021 [42]. nid: not in dataset.
| Gene | Class | Function | Gene name | Gene cluster | Reference | μarray Δbip1/wt | qPCR1 Ap Δbip1/wt | qPCR2 WT Ap/ My | RNAseq WT* Ap/ Gc | EMSA |
|---|---|---|---|---|---|---|---|---|---|---|
| MGG_02420 | Metabolism | Sugar 1,4 lactone oxidase | -1.6 | nid | ||||||
| MGG_02530 | Metabolism | Quinate permease | -1.9 | nid | ||||||
| MGG_02559 | Metabolism | Molybdenum cofactor sulphurase | -1.6 | 2.4 | ||||||
| MGG_03263 | Metabolism | Betaine aldehyde dehydrogenase | BADH2 | [43] | -1.5 | 2.8 | ||||
| MGG_04240 | Metabolism | FAD oxidoreductase | -1.5 | 4.4 | ||||||
| MGG_04738 | Metabolism | Short-chain dehydrogenase | -1.6 | 5.1 | ||||||
| MGG_09681 | Metabolism | Gluconolactonase | -2.4 | 4.3 | ||||||
| MGG_08236 | Secondary Metabolism | Polyketide synthase | -2.2 | 6.3 | ||||||
| MGG_08377 | Secondary Metabolism | O-Methyltransferase | OME1 | ACE1 | [36, 44] | -2.1 | 6.6 | |||
| MGG_08378 | Secondary Metabolism | Cytochrome P450 | CYP4 | ACE1 | [36, 44] | -2.8 | 6.6 | |||
| MGG_08379 | Secondary Metabolism | Cytochrome P450 | CYP3 | ACE1 | [36, 44] | -2.4 | 7.3 | |||
| MGG_08380 | Secondary Metabolism | Enoyl reductase | RAP2 | ACE1 | [36, 44] | -3.8 | -10.0 | 13.3 | 7.4 | Yes |
| MGG_08381 | Secondary Metabolism | Diels-alderase | ORF3 | ACE1 | [36, 44] | -4.1 | -11.3 | 11.1 | 9.4 | Yes |
| MGG_08386 | Secondary Metabolism | Zn finger transcription factor | BC2 | ACE1 | [36, 44] | -1.8 | -5 | 10.8 | 6.6 | No |
| MGG_08387 | Secondary Metabolism | Cytochrome P450 | CYP1 | ACE1 | [36, 44] | -1.8 | 7.1 | |||
| MGG_08391 | Secondary Metabolism | Enoyl reductase | RAP1 | ACE1 | [36, 44] | -2.7 | 7.3 | |||
| MGG_11096 | Secondary Metabolism | Thioesterase | -1.2 | 4.6 | ||||||
| MGG_13405 | Secondary Metabolism | Terpene synthase | -1.3 | 9.7 | ||||||
| MGG_15928 | Secondary Metabolism | Cytochrome P450 | CYP2 | ACE1 | [36, 44] | -1.6 | -3.0 | 11.6 | 10.2 | |
| MGG_02201 | Secreted Enzyme | Peptidase A1 | -4.1 | -9.0 | 11.6 | 8.5 | Yes | |||
| MGG_03771 | Secreted Enzyme | Feruloyl esterase | FAEB | [45] | -2.1 | 8.2 | ||||
| MGG_05855 | Secreted Enzyme | α/β Hydrolase | -1.6 | 4.1 | ||||||
| MGG_08480 | Secreted Enzyme | α/β Hydrolase | -2.6 | 4.5 | ||||||
| MGG_11966 | Secreted Enzyme | Cutinase | -1.6 | 7.8 | ||||||
| MGG_17153 | Secreted Enzyme | Chitinase | -1.6 | 2.3 | ||||||
| MGG_00751 | Secreted Protein | small secreted protein | -1.1 | 1.4 | ||||||
| MGG_03468 | Secreted Protein | Lysm domain protein | SLP2 | [39] | -2.8 | 5.1 | ||||
| MGG_03504 | Secreted Protein | small secreted protein | -1.6 | 1.9 | ||||||
| MGG_05638 | Secreted Protein | small secreted protein | -2 | 8.8 | ||||||
| MGG_06666 | Secreted Protein | small secreted protein | -2.3 | 2.2 | ||||||
| MGG_07934 | Secreted Protein | small secreted protein | -1.8 | 8.0 | ||||||
| MGG_08428 | Secreted Protein | small secreted protein | -2.8 | 7.0 | ||||||
| MGG_09693 | Secreted Protein | Biotrophy assoc.protein 2 | BAS2 | [38] | -2.2 | 2.7 | ||||
| MGG_11610 | Secreted Protein | Biotrophy assoc.protein 3 | BAS3 | [38, 40] | -3.1 | nid | ||||
| MGG_12655 | Secreted Protein | small secreted protein | AVR-PI9 | [37] | -3.2 | 6.8 | ||||
| MGG_17425 | Secreted Protein | small secreted protein | -2.3 | 3.6 | ||||||
| MGG_02160 | Signaling | GPCR PTH11 family, CFEM | -1.2 | -1.1 | 3.4 | 2.2 | ||||
| MGG_03526 | Signaling | N6 Adenine DNA methylase | -1.7 | 3.2 | ||||||
| MGG_03584 | Signaling | GPCR PTH11 family, CFEM | -3.3 | -1.7 | 0.5 | 6.4 | Yes | |||
| MGG_06535 | Signaling | GPCR PTH11 family | -2.9 | -3.9 | 7.2 | 3.8 | Yes | |||
| MGG_10544 | Signaling | GPCR cAMP Glucose receptor-like | -1.7 | 7 | ||||||
| MGG_00545 | Unknown | Unknown | -2.7 | 3.7 |