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(a) RP-HPLC fractionation of semipurified ileal mucosal extract. Antimicrobial fraction 15 eluted from the cation-exchange column (1 M NaCl) was applied to an Aquapore C4 column, and of the eluted fractions, F, G, and H demonstrated antimicrobial activity. (b) AU-PAGE of fraction 15 (lane 1), fraction H (lane 3), and synthetic magainin (lane 2). Of the many positively charged peptides and proteins present in fraction 15, one was purified by RP-HPLC (fraction H in panel a).
