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Dot blot analysis demonstrating the presence of immunoreactive histone H1 in supernatants of detached and cultured (for 18 h) terminal ileal villus epithelial cells. Acid extracts were applied to PVDF membranes, which were subsequently incubated with an anti-human histone H1 monoclonal antibody (b) or control buffer (a). Strong histone H1 immunoreactivity was seen with the monoclonal antibody.
