Table 2.
Association between vaginal micro-flora isolates and Nugent's vaginal flora categories and Amsel's diagnosis of bacterial vaginosis
| Association with Nugent's vaginal flora categories | Association with Amsel's diagnosis of BV | |||||||||
| Isolate | Nugent1 | N2 | %3 | OR4 95% CI | AOR5 95% CI | Amsel6 | N2 | %3 | OR4 95% CI | AOR5 95% CI |
| Lactobacilli | Normal flora | 63 | 74.6 | 1 | 1 | |||||
| Interm. | 54 | 35.2 | 0.18 0.08–0.41 | 0.18 0.08–0.41 | Neg. | 152 | 42.8 | 1 | 1 | |
| BV | 102 | 16.7 | 0.07 0.03–0.15 | 0.06 0.03–0.15 | Pos. | 67 | 26.9 | 0.49 0.26–0.92 | 1.11 0.52–2.38 | |
| P7 | <0.001 | <0.001 | P7 | 0.025 | 0.775 | |||||
| H2O2 - Lactobacilli | Normal flora | 49 | 49.0 | 1 | 1 | |||||
| Interm. | 49 | 14.3 | 0.17 0.07–0.46 | 0.17 0.07–0.47 | Neg. | 134 | 24.6 | 1 | 1 | |
| BV | 97 | 10.3 | 0.12 0.05–0.28 | 0.12 0.05–0.31 | Pos. | 61 | 13.1 | 0.46 0.20–1.07 | 0.94 0.36–2.49 | |
| P7 | <0.001 | <0.001 | P7 | 0.067 | 0.909 | |||||
| G vaginalis | Normal flora | 61 | 11.5 | 1 | 1 | |||||
| Interm. | 54 | 35.2 | 4.19 1.59–11.0 | 3.96 1.50–10.5 | Neg. | 150 | 35.3 | 1 | 1 | |
| BV | 102 | 71.6 | 19.42 7.91–47.6 | 15.45 6.18–38.6 | Pos. | 67 | 68.7 | 4.01 2.17–7.42 | 2.21 1.10–4.44 | |
| P7 | <0.001 | <0.001 | P7 | <0.001 | 0.026 | |||||
| Anaerobic isolates8 | Normal flora | 21 | 0 | 1 | 1 | |||||
| Interm. | 13 | 38.5 | -9 | -9 | Neg. | 43 | 18.6 | 1 | 1 | |
| BV | 31 | 48.4 | -9 | -9 | Pos. | 22 | 54.6 | 5.25 1.68–16.38 | 4.5 1.24–16.37 | |
| P7 | 0.001 | -10 | P7 | 0.003 | 0.018 | |||||
| Mycoplasma hominis | Normal flora | 60 | 13.3 | 1 | 1 | |||||
| Interm. | 53 | 22.6 | 1.90 0.71–5.09 | 1.85 0.69–4.97 | Neg. | 148 | 18.9 | 1 | 1 | |
| BV | 102 | 25.5 | 2.22 0.93–5.29 | 2.00 0.80–4.96 | Pos. | 67 | 26.9 | 1.57 0.80–3.10 | 1.34 0.65–2.76 | |
| P7 | 0.184 | 0.282 | P7 | 0.188 | 0.436 | |||||
1 Nugent's categories: normal flora (score 0–3), intermediate flora (score 4–6), BV (score 7–10).
2 Number of specimens.
3 Percentage positive for the isolate.
4 Crude Odds Ratio.
5 Odds Ratio adjusting for the other method of diagnosing BV.
6 Amsel's diagnosis 'positive' [pos.] if at least 3 out of 4 criteria present (homogenous adherent discharge; pH ≥ 4.6; whiff test positive; clue cells on 20% of epithelial cells); diagnosis is 'negative' [neg.] if <3 criteria present.
7 From X2 test for unadjusted analysis. From likelihood ratio test when adjusting for the other diagnostic method.
8 Anaerobic isolates comprise Prevotella spp, Bacteroides spp, Peptostreptococcus spp and other anaerobes.
9 Not possible to calculate OR relative to normal flora due to lack of anaerobic isolation in normal subjects.
10 Not possible to fit model due to lack of anaerobic isolation in normal subjects.