FIG. 8.

Effects of in vivo administration of anti-IL-15 MAb on recovery of bacteria from the peritoneal cavity and spleen and appearance of γδ T cells in the peritoneal cavity after E. coli infection. C3H/HeN mice were inoculated i.p. with 200 μg of anti IL-15 MAb, or control Ab was injected i.p. 2 h before challenge with 2 × 10⁸ CFU of E. coli. (A) Non-plastic-adherent PEC from infected mice on day 3 were stained with anti-TCR-αβ and anti-TCR-γδ MAbs. (B) The numbers of E. coli CFU recovered from the peritoneal cavities, livers, and spleens of infected mice on day 3 were determined by colony formation assay on tryptic soy agar. Values are means ± standard errors for groups of three mice. Asterisks indicate significant differences from the values for control mice (P < 0.05).