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. 2023 Nov 25;147(2):444–457. doi: 10.1093/brain/awad388

Figure 1.

Figure 1

Qualitative and quantitative evaluation of phosphorylated α-synuclein aggregates in substantia nigra and putamen. Photomicrographs of the mid-substantia nigra (left two columns) and putamen (right two columns) from no motor deficit (NMD; A–D), minimal motor deficits (MMD; E–H), minimal motor deficits with nigral Lewy body (MMD-LB; I–L) and Parkinson’s disease (PD; M–P) show phospho-S129 α-syn (p-S129) staining patterns. p-S129 immunoreactivity was undetectable in the subjects with NMD (A–D) and MMD (E–H). In contrast, p-S129-immunoreactive nigral neurons were observed in subjects with MMD-LB (I and J) and patients with PD (M and N) and p-S129-immunoreactive neurites in putamen were detectable in both subjects with MMD-LB (K and L) and PD (O and P). Scale bar in N = 100 µm in B, F and J, 500 µm in A, E, I and M; 200 µm in C, G, K and O, 20 µm in D, H, L and P. Stereological analyses revealed that no p-S129-positive aggregate in substantia nigra (Q) and putamen (R) was counted in NMD and MMD groups. However, there was a significantly higher densities of p-S129 labelled aggregates in MMD-LB and PD groups. ***P < 0.001 compared with NMD. ##P < 0.01 and ###P < 0.001 compared with MMD.