Figure 3.
Effect of UNC2025 on microglial uptake of α-synuclein preformed fibrils. Microglia were pretreated with vehicle, UNC2025 (3 μm) or cytochalasin D (1 μm) for 1 h, then challenged with pHrodoTM Green-labelled α-synuclein (α-syn) preformed fibrils (PFFs), myelin debris or IgG-opsonized red blood cells (IgG-RBC) for 2 h. (A and B) Quantification of mean green fluorescence intensity (MFI) in induced pluripotent stem cell-derived microglia (iMGL) culture. Paired t-tests were performed; n = 4; *P < 0.05, **P < 0.01. (C) Representative images of iMGL counterstained with Hoechst 33342 (blue). Scale bar = 150 μm. (D) Quantification of mean green fluorescence intensity (MFI) in human primary microglia (hMGL) culture. A paired t-test was performed; n = 5; **P < 0.01. (E) Representative images of hMGL counterstained with Hoechst 33342 (blue). Scale bar = 200 μm.