Fig. 2. Relationship between immune cell phenotype and response.

Mononuclear cells isolated from peripheral blood collected before treatment (C1D1) were analyzed by flow cytometry. Response assessment was performed using RECIST 1.1 and allowed patients to be grouped as those with progressive disease (PD n = 9), stable disease (SD n = 28), and partial or complete response (PR n = 11). A Myeloid-derived suppressor cells (MDSC) (n = 47) were gated as CD45⁺Lin (−) CD33⁺CD14⁻ and CD45⁺Lin(−) CD33⁺HLA-DR⁻). Mean + SEM, two-way ANOVA. B–H Association peripheral T-cell effector status with patients’ responses. Percentages of IFNɣ (n = 43) (B, C) TNFɑ (n = 42) (D–G), RORɣt (n = 45) (I, J) and Foxp3 (n = 43) (K, L) in CD8⁺ or CD4⁺ T cells were analyzed by FACS. Results are expressed as mean + SEM, Kruskal-Wallis ANOVA. Gating started is shown in supplementary Fig. 1A. One representative dot plot from each of the response types is shown. Source data are provided as a Source Data file.