Skip to main content
. 2024 Jan 22;56(1):235–249. doi: 10.1038/s12276-024-01155-9

Fig. 1. CYB5R3 is downregulated in lung cancer.

Fig. 1

a CYB5R3 expression profiles across lung tumor samples (T) and corresponding normal tissues (N) in the TCGA database. The expression data were collected from the TCGA cohort via cBioPortal (http://cbioportal.org) and were then visualized by ggplot2 (version 3.3.6). LUAD; lung adenocarcinoma, LUSC; lung squamous carcinoma. All statistical analyses were performed using R software. b IHC analysis of CYB5R3 in normal lung tissue and lung cancer patient tissue. Representative images of CYB5R3 staining in the tissue array are shown. Scale bar, 100 μm. c CYB5R3 expression levels in normal lung tissues and lung cancer tissues. CYB5R3Low indicates negative (0) or weak (1) IHC staining, and CYB5R3High indicates moderate (2) or strong (3) IHC staining. CYB5R3 expression in two human lung fibroblast and eight lung cancer cell lines. mRNA (d) and protein levels (e) were measured by quantitative RT‒PCR and immunoblot analysis, respectively. f Effects of CYB5R3 siRNA on the protein level of CYB5R3 (upper panel) and cell viability (lower panel) in WI-38 and IMR-90 cells. The values are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01. g H&E staining of lung tissues in CYB5R3+/+ and CYB5R3−/− mice 8 months after IP injections of 0.5 mg/g urethane (n = 3 mice per group). Scale bar, 100 μm.