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. 2024 Jan 22;56(1):235–249. doi: 10.1038/s12276-024-01155-9

Fig. 2. CYB5R3 overexpression induces cell death in lung cancer cells in vitro and in vivo.

Fig. 2

a Cells were infected with EV or CYB5R3 adenovirus at an MOI of 100 for the indicated times, and cell lysates were analyzed by immunoblotting. b Cells were infected with EV or CYB5R3 adenovirus at an MOI (multiplicity of infection) of 100 for 72 h. Cell viability was measured by an SRB assay. The data are presented as the mean ± SD of three independent experiments. c Cleavage of PARP1 and caspase-3 in CYB5R3-infected H1299 and H1703 cells. d Caspase 3/7 activation and annexin staining in CYB5R3-infected H1299 cells. Representative images are shown. Scale bar, 100 μm. e Tumor volume was measured at 2-3-day intervals in xenografted H1299 mice (n = 8 mice/group). f Xenograft tumor weights (n = 8 mice/group). g Body weights (n = 8 mice/group). h CYB5R3 protein level in H1299 xenograft tissues. The data are presented as the means ± SDs. Statistical analysis was performed by two-way ANOVA with post hoc Tukey’s test (e). *P < 0.05, **P < 0.01, ***P < 0.001.