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. 2024 Jan 22;56(1):235–249. doi: 10.1038/s12276-024-01155-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–c RNA-seq analysis in H1299 cells infected with EV (Ad-EV) and CYB5R3 (Ad-CYB5R3). a Heatmap showing the differentially expressed genes (DEGs) from three independent samples treated with CYB5R3 compared with EV or PBS. b KEGG pathway enrichment analysis of the DEGs. The top twenty pathways are shown. c QPCR analysis of the indicated genes in H1299 cells infected with CYB5R3 for 24 h. d Immunoblot analysis of proteins with altered expression in H1299 or H1703 cells infected with CYB5R3 for 24 h. e H1299 cells were transfected with the indicated siRNA and infected with CYB5R3 for 48 h. Cell viability was measured by an SRB assay (lower panel). Immunoblot analysis was performed to measure the indicated protein levels (upper panel). The experiments were repeated three times. The data are shown as the means ± SDs. Student′s t test. **P < 0.01.