Fig. 5.

The NEDD4L/TFRC pathway affects cell damage and ferroptosis. A. CCK-8 was used to detect the cell viability after NEDD4L or TFRC treatment, n = 3. B. The Edu staining was used to detect the cell viability after NEDD4L or TFRC treatment, n = 3. C. The TUNEL staining was used to detect the cell apoptosis after NEDD4L or TFRC treatment, n = 3. D. The contents of Fe 2 + in HT-22 of each treatment group were detected. E. The contents of GSH in HT-22 of each treatment group were detected. F. The contents of ROS in HT-22 of each treatment group were detected. G. Confocal imaging of BODIPYC11-ROS was performed in HT-22 cells treated with NEDD4L or TFRC. H. QRT-PCR was used to detect the expression levels of ferroptosis markers in HT-22 of each treatment group. n = 3. I. WB was used to detect the expression levels of ferroptosis markers in HT-22 of each treatment group. n = 3. * vs NC + OGD/R group P < 0.05, # vs NEDD4L + OGD/R group P < 0.05, & vs TFRC + OGD/R group P < 0.05