Fig. 6.

METTL3 promotes the mRNA stability of NEDD4L. A. RM2Target and SRAMP data were used to predict the methylation sites of NEDD4L. B–C. ELISA was used to detect the expression level of m6A in MCAO model and HT-22 cells. D. NEDD4L degradation in si-NC (siRNA-NC was transfected) group and si-METTL3 (siRNA-METTL3 was transfected) group after cycloheximide treatment. E. The RIP analysis showing the enrichment of NEDD4L on IgG and METTL3 in the NEDD4L-WT and NEDD4L-MUT. F The methylated RNA immunoprecipitation (meRIP) was used to verify the effect of inhibition of METTL3 on NEDD4L m6A levels. Each assay was repeated three times independently. n = 3. * vs si-NC group or Con group P < 0.05