Fig. 6.

Propionate suppresses LPS-induced inflammation in RAW264.7 macrophages via the JAK2-STAT3 signaling pathway. (A) KEGG analysis of differentially enriched pathways by propionate. (B) Fold change of selected genes by RNA-Seq and the correlation analysis of selected genes between the RT-qPCR and RNA-Seq results. Western blot analysis of p-JAK2 (C) and p-STAT3 (G) as well as the relative mRNA expression of pro-inflammatory cytokines (D-F, H-J). IL-1β, interleukin-1β; iNOS, inducible nitric oxide synthase; IL-6, interleukin-6. *** P< 0.001.