Fig. 2.
Performances of meHOLMES.
(A) Fluorescence intensities released by Cas12a trans-cleavage were collected at 30 min (mean ± SD, n = 3). (B) Correlation of the fluorescence intensities and the ratios of methylated DNA sequences. The solid line was a linear regression line (R2 = 0.98; n = 3). (C) Fluorescence intensities of the meHOLMES system with different target sequences. Fluorescence intensities at 30 min were shown (mean ± SD, n = 3, two-tailed Student's t-test, ****p < 0.0001). (D) Intuitive results using the LFA-assisted meHOLMES system.