Skip to main content
. 2024 Jan 4;56(1):156–167. doi: 10.1038/s12276-023-01135-5

Fig. 2. WTAP overexpression aggravated the TNF-α-induced osteoarthritic chondrocyte phenotype.

Fig. 2

a–d Overexpression efficiency of WTAP in human chondrocytes. Relative WTAP expression measured by qRT‒PCR (a), Western blotting (b), quantitative analysis (c), and IF staining (d) in chondrocytes after transfection with flag-WTAP. Scale bars: 100 µm. e The m6A level of total RNA in chondrocytes with or without WTAP overexpression. f Relative mRNA expression of ADAMTS4, ADAMTS5, MMP13, IL-6, IL-8, and iNOS was measured by qRT‒PCR in chondrocytes with or without WTAP overexpression. g, h Western blotting (g) and quantitative analysis (h) of ADAMTS4, ADAMTS5, and MMP13 expression in chondrocytes with or without WTAP overexpression. i, j Detection of ROS (i) and quantitative analysis (j) in chondrocytes with or without WTAP overexpression. Scale bars: 200 µm. Data are presented as the means ± SEMs. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.