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. 2024 Jan 4;56(1):156–167. doi: 10.1038/s12276-023-01135-5

Fig. 5. The FRZB-Wnt/β-catenin axis was regulated by WTAP in chondrocytes.

Fig. 5

ac Protein levels of FRZB and β-catenin were analyzed in chondrocytes with or without WTAP overexpression. d, e Relative mRNA expression levels of FRZB (d) and β-catenin (e) were measured by qRT‒PCR in chondrocytes with or without WTAP overexpression. f, g IF staining of intracellular FRZB (f) and β-catenin (g) in TNF-α-pretreated cells with or without WTAP overexpression. Scale bars: 100 µm. hj Protein levels of FRZB and β-catenin were analyzed in TNF-α-pretreated chondrocytes with or without KO-WTAP transfection. k, l Relative mRNA expression levels of FRZB (k) and β-catenin (l) were measured by qRT‒PCR in TNF-α-pretreated cells with or without KO-WTAP transfection. m, n IF staining of intracellular FRZB (m) and β-catenin (n) expression in TNF-α-pretreated cells with or without KO-WTAP transfection. Scale bars: 100 µm. Data are presented as the means ± SEMs. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant.