Fig. 3.

(A) Schematic representation of TIF collection by centrifugation from resected tumors i.t. injected with AuGSH (1×, 300 µg in PBS buffer pH 7.4), AuPC (1×, 300 µg in PBS buffer pH 7.4), and ICG (5 mM in DI-water). (B) Ex vivo NIR-II imaging of resected tumors before and after centrifugation (n = 3). The imaging was done under an 808-nm laser excitation at a power density of 70 mW/cm² using an 1,100-nm long-pass filter, 5× objective, scale bar is 2 mm. The exposure times were 5 and 3 ms for AuPC/AuGSH and ICG, respectively. (C) Color photographs (Top) of collected TIF extracts inside Eppendorf vials. NIR-II images of collected extracts (Bottom, 10× diluted solutions, 3 ms-exposure time). (D) Tumor MFI difference before and after the centrifugation. (E) The calculated percentages of NIR-II brightness in collected extracts (10× diluted solutions, 3 ms-exposure time). Error bars represent SD of three repeated experiments.