Fig. 3.

Effects of LXB4 and RvE1 on the cilia beat phenotype. LXB4 (10 nM) and RvE1 (10 nM) or vehicle (ethanol) were added, 24 h after mucus accumulation, into the basolateral compartment of culture inserts from 4 (for LXB4 experiment) and 5 (for RvE1 experiment) CF patients. Videos were acquired at different times after LXB4 exposure: at 1 h (35 videos with vehicle and 16 with LXB4), at 24 h (39 with vehicle and 26 with LXB4), and at 48 h (20 with vehicle and 15 with LXB4). Similarly, videos were acquired at different times after RvE1exposure: at 1 h (58 with vehicle and 46 with RvE1), at 24 h (59 with vehicle and 49 with RvE1), and at 48 h (39 with vehicle and 32 with RvE1). The scatter plot on the first two PC is displayed, each point corresponding to one video acquisition. PERMANOVA statistical tests were performed to compare the cilia beat phenotype (centroid) recorded on CF hNEC exposed to the vehicle (blue) and CF hNEC exposed to SPMs (green), with P-values >0.5 considered as not significant (NS). PCA analysis from non-CF samples exposed to the vehicle is displayed in gray.