Fig. 6.

RvE1 modified mucins secretion and structure. (A) Representative confocal projection images of MUC5AC (green) and MUC5B (red) secreted apically by non-CF and CF hNECs cultures treated with vehicle solution or RvE1 (10 nM) ± Ani9 (10 µM). Culture inserts were fixed after 48 h of treatment. Orthogonal views (Z) of MUC5AC. (White Scale bar: 10 μm.) (B) Average thickness of MUC5AC structures measured on hNEC from two CF donors and four non-CF donors (two culture inserts by condition and three to five acquisitions). (C) Mean transcription levels of MUC5B and MUC5AC in untreated hNEC from eight non-CF donors and six CF donors and in hNEC derived from four CF donors and treated for 24 h with RvE1 (10 nM). Transcription levels are expressed as delta Ct (Ct of the mucin minus Ct of the housekeeping gene). Mean ± SEM; t test; *P < 0.05 and ****P < 0.0001.