Fig. 1.

Met improved H2O2-induced apoptosis of NIT-1 cells. (A) Calcein AM/PI staining result of NIT-1 cells. Green fluorescence corresponding to living cells; red fluorescence is marked by a yellow arrow due. Scale bar = 50 μm. (B) Representative images of apoptotic NIT-1 cells based on annexin V/PI staining. The histogram refers to the apoptotic NIT-1 cells rate (n = 3). (C) The panel and histogram correspond to the relative levels of apoptosis-associated proteins, the internal control was β-actin (n = 3). ^bP < 0.05, ^eP < 0.05, compare to the control and H2O2 group, respectively.