Fig. 3.

Apoptosis of NIT-1 cells induced by METTL14 silencing. (A–B) The silencing efficiency of si-METTL14-1, 2 and 3 was tested based on qRT-PCR and WB method (n = 3). (C) Calcein AM/PI staining result of the relevant silenced NIT-1 cells, Scale bar = 50 μm. (D) Representative images of apoptotic staining NIT-1 cells, the histogram corresponds to apoptotic NIT-1 cells (n = 3). (E) The panel and histogram refer to relative levels of apoptosis-related proteins (n = 3). ^bP < 0.05, compared to siNC group; ^eP < 0.05, to si-METTL14-1 group.