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Purification of Manilkara zapota seed Lectin.
| Purification step | Total protein (mg) | Total activity (HU) | Specific activity (HU/mg) | Purification Fold |
% of Yield (Per step) |
|---|---|---|---|---|---|
| Crude extract | 30,000 | 7,20,000 | 24 | 1 | 100 |
| Ammonium sulphate precipitation | 600 | 96,000 | 160 | 6.66 | 13.33 |
| Affinity chromatography | 19.5 | 8192 | 420 | 17.5 | 1.13 |
The given data is calculated for 100 g seeds. Mice RBCs were used for the hemagglutination assay.
