SPI-4 is present in different ETs of S. enterica serovars. A Southern blot was generated from 10 of the most common ET S. enterica serovars and hybridized with a DNA probe generated from SPI-4 (Fig. 5). Lanes: 1, Dublin; 2, Enteritidis; 3, Gallinarum; 4, Minnesota; 5, Paratyphi A; 6, Paratyphi B; 7, Paratyphi C; 8, Pullorum; 9, Typhi, 10, Typhimurium.