FIG. 3.

Nucleotide sequence alignments at the boundaries of SPI-4. The DNA sequences of the ssb boundary (A) and the yjcb_Ecoli boundary (B) of SPI-4 (ST) are aligned with the corresponding sequences of the E. coli chromosomal region (EC). Alignments were performed with the MacVector program. The numbering of the nucleotides of SPI-4 corresponds to the 27,290-bp DNA sequence determined in this study. A DNA sequence that forms a tRNA-like structure is boxed and shown in boldface letters in panel A. The hairpin structure is indicated by a pair of inverted arrows in panel B. The numbering of the E. coli sequences is from the published sequence (accession no. ECAE000479). Single-letter symbols are used for the amino acid sequences of Ssb and Yjcb_Ecoli and protein R shown beneath the nucleotide sequences. yjcb_Sty, a hypothetical 13-kDa protein.