TABLE 1.
Summary of sequence analysis of putative proteins encoded by SPI-4
| Protein | No. of amino acids (predicted mass [kDa]) | Characteristics |
|---|---|---|
| A | 524 (58) | Has five transmembrane helices with one at the N terminus |
| B | 121 (14) | Unknown |
| C | 439 (51) | Shares significant homology with aggA gene product (22% identity, 45% similarity), hasF gene product (18% identity, 45% similarity), prtF gene product (21% identity, 43% similarity), and cyaE gene product (19% similarity, 43% similarity); has one transmembrane helix at N terminus; belongs to ABC exporter outer membrane component family |
| D | 425 (48) | Shares significant homology with lipC gene product (24% identity, 46% similarity), prtE gene product (25% identity, 45% similarity), hlyD gene product (23% identity, 47% similarity), and lktD gene product (21% identity, 43% similarity); has one transmembrane helix at N terminus; belongs to HlyD family of secretion proteins with periplasmic domains |
| E | 130 (14) | Has one transmembrane helix at N terminus |
| F | 526 (56) | Shares significant homology with a 93.4-kDa hypothetical protein of A. calcoaceticus (27% identity and 45% similarity over a stretch of 444 amino acids) |
| G | 431 (46) | Shares significant homology with a 93.4-kDa hypothetical protein of A. calcoaceticus (27% identity and 43% similarity over a stretch of 396 amino acids); has one transmembrane helix |
| H | 116 (13) | Unknown |
| I | 754 (81) | Shares significant homology with part of a hypothetical 308.8-kDa protein of Synechocystis sp. strain PCC6803 (31% identity and 44% similarity over a stretch of 724 amino acids) |
| J | 172 (19) | Unknown |
| K | 820 (88) | Shares significant homology with part of a hypothetical 308.8-kDa protein of Synechocystis sp. strain PCC6803 (32% identity and 46% similarity over a stretch of 780 amino acids) |
| L | 206 (22) | ORF_M was inserted by Tn10 into strain MST2097, which is unable to survive within macrophage |
| M | 123 (13) | Unknown |
| N | 92 (9.6) | Unknown |
| O | 1,512 (162) | Shares significant homology with part of a hypothetical 308.8-kDa protein of Synechocystis sp. strain PCC6803 (25% identity and 42% similarity over a stretch of 1,327 amino acids); has three transmembrane helices with one at the C terminus |
| P | 463 (49) | Unknown |
| Q | 38 (4.5) | Unknown |
| R | 598 (67) | Shares significant homology with lktB gene product (28% identity, 51% similarity), cylB gene product (27% identity, 52% similarity), hlyB gene product (27% identity, 50% similarity), apxIB gene product (26% identity, 50% similarity); has five transmembrane helices (N terminus outside); belongs to ABC transporter family with ATP-GTP-binding site motif A |