TABLE 2.

Genetic analysis of avirulent mutants^a

Mutant	β-Galactosidase activity				Species specificityb	Map position (min)	Sequence homology	Reference
	LBc	MinGluc,d	DMEMe	J774e
CL13	−	NG	+	+		99	deoA	Blattner et al. (5)
CL79	+	+	+	+	Salmonella	pVstm	3′ ORF11	Friedrich et al. (14)
CL230	+	+	+	+	Salmonella	80
CL448	+	+	+	+		17	tolB	Levengood et al. (20)
CL98	−	−	−	−		94	5′ phoN	Blattner et al. (5)
CL238	−	−	−	−		25	o85	Blattner et al. (5)
CL287	−	+	−	+		IC	yabJ/K	Blattner et al. (5)
CL288	+	+	+	+		97	mdoB	Blattner et al. (5)
CL401	−	−	−	+		51	fmt	Blattner et al. (5)
CL500	−	+	+	+		34	pdx	Blattner et al. (5)
CL547	−	+	−	+		70	yhdJ	Blattner et al. (5)
CL554	+	+	+	+		54	mdh	Blattner et al. (5)
14028	−	−	−	−	NA	NA	NA

^aNA, not applicable; NG, no growth; IC, inconclusive. 

^bMutants which hybridized only with Salmonella are indicated. The remainder hybridized with at least one other enterobacterial strain. 

^cGrowth on agar containing X-Gal, scored as blue colonies. Results were similar for aerobic and anaerobic conditions. 

^dMinGlu, minimal glucose medium. 

^eGrowth after contact with J774.10 macrophages or DMEM tissue culture medium, scored as blue wells.