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Effects of isogenic CNF1+ and CNF1−, hemolytic E. coli on survival of T24 bladder cell monolayers. T24 bladder cell monolayers were inoculated with E. coli F3, F3.297 (cnf1::pSE297), F11, or F11.297 (cnf1::pSE297). The fraction of the T24 monolayer remaining was determined at 1-h intervals by staining with the dye methylene blue. Data for each strain are means ± standard errors from two or three experiments.
