Figure 1.

(A) Representative liquid chromatography-tandem mass spectrometry (LC-MS/MS) chromatograms of hydroxytyrosol and its metabolites in plasma obtained 30 min after the oral administration to Sprague-Dawley rats of Arbequina table olives containing hydroxytyrosol at 2.95 mg/kg (30 olives group). (B) Multiple reaction monitoring (MRM) parameters corresponding to hydroxytyrosol and its metabolites set or obtained by LC-MS/MS. The quantification (Q) and qualification (I) transitions were monitored for the hydroxytyrosol and its metabolites while only one transition was employed for the internal standard (I.S.). DP, declustering potential; EP, entrance potential; CE, collision energy; CXP, collision cell exit potential.