KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| Rabbit anti-Parvalbumin | Abcam | Cat# ab11427, RRID:AB_298032 |
| Mouse anti-Parvalbumin | Sigma | Cat# P3088, RRID:AB_477329 |
| Rabbit anti-Somatostatin 28 | Abcam | Cat# ab111912, RRID:AB_10903864 |
| Rabbit anti-μOR | Abcam | Cat# ab134054, RRID:AB_3122135 |
| Rabbit anti-Satb1 | Abcam | Cat# ab109122, RRID:AB_10862207 |
| Rabbit anti-pIκBα | Cell Signaling Technology | Cat# 2859; RRID: AB_561111 |
| Chicken anti-GFP | Aves Labs | Cat# GFP-1020, RRID:AB_10000240 |
| Rabbit anti-GFP | Abcam | Cat# ab290, RRID:AB_303395 |
| Guinea Pig anti-RFP | Synaptic Systems | Cat# 390005, RRID:AB_2737051 |
| Rabbit anti-RFP | Antibodies-Online | Cat# ABIN129578, RRID:AB_10781500 |
| AF488 Goat anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# A-11008, RRID:AB_143165 |
| AF488 Goat anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# A-11034, RRID:AB_2576217 |
| AF488 Donkey anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# A-32790, RRID:AB_2762833 |
| CF488A Donkey anti-Mouse IgG (H+L) | Biotium | Cat# 20014, RRID:AB_10561327 |
| CF488A Donkey anti-Rabbit IgG (H+L) | Biotium | Cat# 20015, RRID:AB_10559669 |
| CF488A Donkey anti-Chicken IgY (IgG) (H+L) | Biotium | Cat# 20166, RRID:AB_10854387 |
| AF555 Goat anti-Rabbit IgG (H+L) | Thermo Fisher Scientific | Cat# A-21429, RRID:AB_2535850 |
| AF555 Goat anti-Guinea Pig IgG (H+L) | Thermo Fisher Scientific | Cat# A-21435, RRID:AB_2535856 |
| CF555 Donkey anti-Rabbit IgG (H+L) | Biotium | Cat# 20038, RRID:AB_10558011 |
| CF555 Donkey anti-Guinea Pig IgG (H+L) | Biotium | Cat# 20276-1, RRID:AB_10853597 |
| AF594 Donkey anti-Guinea Pig IgG (H+L) | Thermo Fisher Scientific | Cat# A-11076, RRID:AB_141930 |
| CF647 Donkey anti-Mouse IgG (H+L) | Biotium | Cat# 20046-1, RRID:AB_10853778 |
| CF647 Donkey anti-Rabbit (H+L) | Biotium | Cat# 20047, RRID:AB_10559808 |
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| Bacterial and virus strains | ||
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| pAAV(PHP.eB)-BiPVe4-ChR2-mCherry | Addgene64 | RRID:Addgene_213937 |
| pAAV(PHP.eB)-BiPVe4-tdTom-tdTomnls | Addgene64 | RRID:Addgene_213936 |
| pAAV(AAV9)-Ef1a-DIO-C1V1(t/t)-TS-mCherry | Addgene | RRID:Addgene_100061-AAV9 |
| pAAV(PHP.eB)-S5E2-ChR2-mCherry | Addgene44 | RRID:Addgene_135634-PHPeB |
| pAAV(AAV5)-pCAG-FLEX-EGFP-WPRE | Addgene101 | RRID:Addgene_51502-AAV5 |
|
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| Chemicals, peptides, and recombinant proteins | ||
|
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| DAMGO | Tocris | Cat# 1171 |
| CTAP | Tocris | Cat# 1560 |
| DPDPE | Tocris | Cat# 1431 |
| Naltrindole hydrochloride | Millipore Sigma | Cat# N115 |
| Morphine Sulfate Injection, USP (4 mg/mL) | Hikma Pharmaceuticals | NDC: 0641-6125 |
| Naloxone Hydrochloride Injection (0.4 mg/mL) | Mylan Institutional | NDC: 67457-292-02 |
| DNQX disodium salt | Tocris | Cat# 2312 |
| DL-APV | Tocris | Cat# 0105 |
| CGP 55845 hydrochloride | Tocris | Cat# 1248 |
| ω-Agatoxin IVA | Tocris | Cat# 2799 |
| ω-Conotoxin GVIA | Tocris | Cat# 1085 |
| WIN 55212-2 mesylate | Tocris | Cat# 1038 |
| Biocytin | Millipore Sigma | Cat# B4261 |
| AF488 streptavidin | Thermo Fisher Scientific | Cat# S11223 |
| AF555 streptavidin | Thermo Fisher Scientific | Cat# S21381 |
| DAPI | Millipore Sigma | Cat# D9542 |
| RNAscope mouse probe Mm-Pvalb | Advanced Cell Diagnostic | Cat# 421931 |
| RNAscope mouse probe Mm-Oprm1-C2 | Advanced Cell Diagnostic | Cat# 315841-C2 |
| RNAscope mouse probe Mm-Oprd1 | Advanced Cell Diagnostic | Cat# 427371 |
| RNAscope macaque probe Mmu-PVALB | Advanced Cell Diagnostic | Cat# 461691 |
| RNAscope macaque probe Mmu-OPRM1-C2 | Advanced Cell Diagnostic | Cat# 518941-C2 |
| EdU | Thermo Fisher Scientific | Cat# A10044 |
| Opal 520 | AKOVA | Cat# OP-001001 |
| Opal 570 | AKOVA | Cat# OP-001003 |
| ProLong Diamond Antifade Mountant with DAPI | Thermo Fisher Scientific | Cat# P36962 |
| ProLong Gold Antifade Mountant | Thermo Fisher Scientific | Cat# P10144 |
| GTPγS-Li4 | Sigma | Cat# G8634 |
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| Critical commercial assays | ||
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| Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 | 10x Genomics | Cat# PN-1000075 |
| RNAscope Multiplex Fluorescent v2 Assay | Advanced Cell Diagnostic | Cat# 323100 |
| RNeasy Plus Micro Kit | Qiagen | Cat# 74034 |
| RNA 6000 Pico kit | Agilent | Cat# 5067-1513 |
| SMARTer Stranded Total RNA-Seq. Kit v2 | Takara Bio | Cat# 634411 |
| Click-iT EdU imaging Kit | Thermo Fisher Scientific | Cat# C10340 |
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| Experimental models: Organisms/strains | ||
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| Mouse: PVCre/Cre | Jax102 | RRID:IMSR_JAX:017320 |
| Mouse: SSTCre/Cre | Jax103 | RRID:IMSR_JAX:013044 |
| Mouse: Tac1Cre/Cre | Jax104 | RRID:IMSR_JAX:021877 |
| Mouse: Nkx2.1Cre/Cre | Jax105 | RRID:IMSR_JAX:008661 |
| Mouse: Dlx5/6Cre/Cre | Jax106 | RRID:IMSR_JAX:008199 |
| Mouse: tdTomato(Ai9)fl/fl | Jax107 | RRID:IMSR_JAX:007909 |
| Mouse: ChR2(Ai32)-eYFPfl/fl | Jax108 | RRID:IMSR_JAX:024109 |
| Mouse: ArchT(Ai40D)-eGFPfl/fl | Jax109 | RRID:IMSR_JAX:021188 |
| Mouse: Rpl22(RiboTag)HA/HA | Jax48 | RRID:IMSR_JAX:011029 |
| Mouse: CAG-Sun1/sfGFPfl/fl | Jax110 | RRID:IMSR_JAX:030952 |
| Mouse: Oprm1fl/fl | Jax | RRID:IMSR_JAX:030074 |
| Mouse: μOR-mCherry | Jax35 | RRID:IMSR_JAX:029013 |
| Mouse: PV-tdTomato | Jax | RRID:IMSR_JAX:027395 |
| Mouse: C57BL/6J | Jax | RRID:IMSR_JAX:000664 |
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| Software and algorithms | ||
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| pClamp 10.7 | Molecular Devices | RRID:SCR_011323 |
| FIJI (ImageJ) | NIH | RRID:SCR_002285 |
| Excel | Microsoft | RRID:SCR_016137 |
| Prism | Graphpad | RRID:SCR_002798 |
| ZEN Microscopy Software | Zeiss | RRID:SCR_013672 |
| SPOT Basic 5.2 | SPOT Imaging | RRID:SCR_014313 |
| CellRanger v7.0.0 | 10x Genomics | |
| DESeq2 | Bioconductor111 | RRID:SCR_015687 |
| FIJI Macro: colocIHC | this manuscript | https://doi.org/10.5281/zenodo.10063200 |