|
Antibodies
|
| Rabbit anti-Parvalbumin |
Abcam |
Cat# ab11427, RRID:AB_298032 |
| Mouse anti-Parvalbumin |
Sigma |
Cat# P3088, RRID:AB_477329 |
| Rabbit anti-Somatostatin 28 |
Abcam |
Cat# ab111912, RRID:AB_10903864 |
| Rabbit anti-μOR |
Abcam |
Cat# ab134054, RRID:AB_3122135 |
| Rabbit anti-Satb1 |
Abcam |
Cat# ab109122, RRID:AB_10862207 |
| Rabbit anti-plKBα |
Cell Signaling Technology |
Cat# 2859, RRID:AB_561111 |
| Chicken anti-GFP |
Aves Labs |
Cat# GFP-1020, RRID:AB_10000240 |
| Rabbit anti-GFP |
Abcam |
Cat# ab290, RRID:AB_303395 |
| Guinea Pig anti-RFP |
Synaptic Systems |
Cat# 390005, RRID:AB_2737051 |
| Rabbit anti-RFP |
Antibodies-Online |
Cat# ABIN129578, RRID:AB_10781500 |
| AF488 Goat anti-Rabbit IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-11008, RRID:AB_143165 |
| AF488 Goat anti-Rabbit IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-11034, RRID:AB_2576217 |
| AF488 Donkey anti-Rabbit IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-32790, RRID:AB_2762833 |
| CF488A Donkey anti-Mouse IgG (H+L) |
Biotium |
Cat# 20014, RRID:AB_10561327 |
| CF488A Donkey anti-Rabbit IgG (H+L) |
Biotium |
Cat# 20015, RRID: AB_10559669 |
| CF488A Donkey anti-Chicken IgY (IgG) (H+L) |
Biotium |
Cat# 20166, RRID: AB_10854387 |
| AF555 Goat anti-Rabbit IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-21429, RRID:AB_2535850 |
| AF555 Goat anti-Guinea Pig IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-21435, RRID:AB_2535856 |
| CF555 Donkey anti-Rabbit IgG (H+L) |
Biotium |
Cat# 20038, RRID:AB_10558011 |
| CF555 Donkey anti-Guinea Pig IgG (H+L) |
Biotium |
Cat# 20276-1, RRID:AB_10853597 |
| AF594 Donkey anti-Guinea Pig IgG (H+L) |
Thermo Fisher Scientific |
Cat# A-11076, RRID:AB_141930 |
| CF647 Donkey anti-Mouse IgG (H+L) |
Biotium |
Cat# 20046-1, RRID:AB_10853778 |
| CF647 Donkey anti-Rabbit (H+L) |
Biotium |
Cat# 20047, RRID:AB_10559808 |
|
Bacterial and virus strains
|
| pAAV(PHP.eB)-BiPVe4-ChR2-mCherry |
Addgene60
|
RRID:Addgene_213937 |
| pAAV(PHP.eB)-BiPVe4-tdTom-tdTomnls
|
Addgene60
|
RRID:Addgene_213936 |
| pAAV(AAV9)-Ef1 a-DIO-C1V1 (t/t)-TS-mCherry |
Addgene |
RRID:Addgene_100061-AAV9 |
| pAAV(PHP.eB)-S5E2-ChR2-mCherry |
Addgene62
|
RRID:Addgene_135634-PHPeB |
| pAAV(AAV5)-pCAG-FLEX-EGFP-WPRE |
Addgene99
|
RRID:Addgene_51502-AAV5 |
|
Chemicals, peptides, and recombinant proteins
|
| DAMGO |
Tocris |
Cat# 1171 |
| CTAP |
Tocris |
Cat# 1560 |
| DPDPE |
Tocris |
Cat# 1431 |
| Naltrindole hydrochloride |
Millipore Sigma |
Cat# N115 |
| Morphine Sulfate Injection, USP (4 mg/mL) |
Hikma Pharmaceuticals |
NDC: 0641-6125 |
| Naloxone Hydrochloride Injection (0.4 mg/mL) |
Mylan Institutional |
NDC: 67457-292-02 |
| DNQX disodium salt |
Tocris |
Cat# 2312 |
| DL-APV |
Tocris |
Cat# 0105 |
| CGP 55845 hydrochloride |
Tocris |
Cat# 1248 |
| ω-Agatoxin IVA |
Tocris |
Cat# 2799 |
| ω-Conotoxin GVIA |
Tocris |
Cat# 1085 |
| WIN 55212-2 mesylate |
Tocris |
Cat# 1038 |
| Biocytin |
Millipore Sigma |
Cat# B4261 |
| AF488 streptavidin |
Thermo Fisher Scientific |
Cat# S11223
|
| AF555 streptavidin |
Thermo Fisher Scientific |
Cat# S21381 |
| DAPI |
Millipore Sigma |
Cat# D9542 |
| RNAscope mouse probe Mm-Pvalb |
Advanced Cell Diagnostic |
Cat# 421931 |
| RNAscope mouse probe Mm-Oprm1-C2 |
Advanced Cell Diagnostic |
Cat# 315841-C2 |
| RNAscope mouse probe Mm-Oprd1 |
Advanced Cell Diagnostic |
Cat# 427371 |
| RNAscope macaque probe Mmu-PVALB |
Advanced Cell Diagnostic |
Cat# 461691 |
| RNAscope macaque probe Mmu-OPRM1-C2 |
Advanced Cell Diagnostic |
Cat# 518941-C2 |
| EdU |
Thermo Fisher Scientific |
Cat# A10044 |
| Opal 520 |
AKOVA |
Cat# OP-001001 |
| Opal 570 |
AKOVA |
Cat# OP-001003 |
| ProLong Diamond Antifade Mountant with DAPI |
Thermo Fisher Scientific |
Cat# P36962
|
| ProLong Gold Antifade Mountant |
Thermo Fisher Scientific |
Cat# P10144
|
| GTPγS-Li4
|
Sigma |
Cat# G8634 |
|
Critical commercial assays
|
| Chromium Single Cell 3’ GEM, Library & Gel Bead Kit v3 |
10x Genomics |
Cat# PN-1000075 |
| RNAscope Multiplex Fluorescent v2 Assay |
Advanced Cell Diagnostic |
Cat# 323100 |
| RNeasy Plus Micro Kit |
Qiagen |
Cat# 74034 |
| RNA 6000 Pico kit |
Agilent |
Cat# 5067-1513 |
| SMARTer Stranded Total RNA-Seq. Kit v2 |
Takara Bio |
Cat# 634411 |
| Click-iT EdU imaging Kit |
Thermo Fisher Scientific |
Cat# C10340
|
|
Experimental models: Organisms/strains
|
| Mouse: PVCre/Cre
|
Jax100
|
RRID:IMSR_JAX:017320 |
| Mouse: SSTCre/Cre
|
Jax101
|
RRID:IMSR_JAX:013044 |
| Mouse: Tac1Cre/Cre
|
Jax102
|
RRID:IMSR_JAX:021877 |
| Mouse: Nkx2.1Cre/Cre
|
Jax103
|
RRID:IMSR_JAX:008661 |
| Mouse: Dlx5/6Cre/Cre
|
Jax104
|
RRID:IMSR_JAX:008199 |
| Mouse: tdTomato(Ai9)fl/fl
|
Jax105
|
RRID:IMSR_JAX:007909 |
| Mouse: ChR2(Ai32)-eYFPfl/fl
|
Jax106
|
RRID:IMSR_JAX:024109 |
| Mouse: ArchT(Ai40D)-eGFPfl/fl
|
Jax107
|
RRID:IMSR_JAX:021188 |
| Mouse: Rpl22(RiboTag)HA/HA
|
Jax44
|
RRID:IMSR_JAX:011029 |
| Mouse: CAG-Sun1/sfGFPfl/fl
|
Jax108
|
RRID:IMSR_JAX:030952 |
| Mouse: Oprm1fl/fl
|
Jax |
RRID:IMSR_JAX:030074 |
| Mouse: μOR-mCherry |
Jax31
|
RRID:IMSR_JAX:029013 |
| Mouse: PV-tdTomato |
Jax |
RRID:IMSR_JAX:027395 |
| Mouse: C57BL/6J |
Jax |
RRID:IMSR_JAX:000664 |
|
Software and algorithms
|
| pClamp 10.7 |
Molecular Devices |
RRID:SCR_011323 |
| FIJI (ImageJ) |
NIH |
RRID:SCR_002285 |
| Excel |
Microsoft |
RRID:SCR_016137 |
| Prism |
Graphpad |
RRID:SCR_002798 |
| ZEN Microscopy Software |
Zeiss |
RRID:SCR_013672 |
| SPOT Basic 5.2 |
SPOT Imaging |
RRID:SCR_014313 |
| CellRanger v7.0.0 |
10x Genomics |
|
| DESeq2 |
Bioconductor109
|
RRID:SCR_015687 |
| FIJI Macro: colocIHC |
this manuscript |
https://doi.org/10.5281/zenodo.10063200
|
