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[Preprint]. 2024 Jan 18:rs.3.rs-3871185. [Version 1] doi: 10.21203/rs.3.rs-3871185/v1

Figure 2. Arid1a is required for induction and maintenance of GCs.

Figure 2

A. Representative flow cytometry analysis of splenic GC B cells from NP-Ova immunized Arid1a fl/fl, Cg1 ere and Cg1 Arid1a KO mice at 14 days post-immunization. B. Quantification of GC B cell frequency in spleen of Control (Ctrl) (both Arid1a fl/fl and Cg1 cre)and Cg1 Arid1a KO mice. C. Quantification of ELISA for detection of NP-specific low affinity (using NP-36) and high affinity (using NP-2) IgM and IgG1 antibodies in the sera of NP-Ova immunized Ctrl (Arid1a fl/fl and Cg1 cre; (n=6)) and Cg1 Arid1a KO (n=6). The serum antibodies were measured with 3-fold serial dilutions. Sera were collected prior to immunization Day 0 (D0) and at 14 days post-immunization (D14). D. Representative flow cytometry analysis of splenic GC B cells from SRBC immunized Arid1a fl/fl, CD19 Arid1a KO and Cg1 Arid1a KO mice at day 14 post-immunization. E. Quantification of GC B cell frequency in spleen of Arid1a fl/fl, CD19 Arid1a KO and Cg1 Arid1a KO mice. Statistical significance is calculated using two-tailed Student’s t-test for C, multiple unpaired t-tests for D and one-way ANOVAfor G. Error bars represent mean ± s.e.; *p value ≤ 0.05; **p value ≤ 0.01;***p value ≤ 0.0005; ****p value < 0.0001.