Figure 4.

Gene expression patterns in cultured human myoblasts with internalized patient antibodies. Standardized expression levels (Z-scores) of the top 20 anti-Mi2-specific genes, the top 20 anti-PM/Scl-specific genes, and the top 20 IFNB1-specific genes are shown for cultured human myoblasts electroporated with purified antibodies from individual myositis patients and healthy controls. Anti-Mi2-specific genes are overexpressed predominantly in myoblasts electroporated with antibodies from anti-Mi2-positive patients. Anti-PM/Scl-specific genes are overexpressed exclusively in myoblasts electroporated with antibodies from anti-PM/Scl-positive patients. IFNB1-specific genes are most highly expressed in myoblasts electroporated with antibodies from 3 of 5 anti-MDA5-positive patients.