TABLE 1.
Immunization schemea
| Protein (Aotus monkey) | Peptide or compound | Sequence |
|---|---|---|
| LSA1 (M21) | LSA1-J/Lipo | ERRAKEKLQEQQSDLEQRKADTKK K(Pam)-NH2b |
| LSA1-NR | DTKKNLERKKEHGDILAEDLYGRLEIP | |
| LSA1-REP | LAKEKLQEQQSDLEQERLAKEKLQEQQSDLEQERLAKEKLQ | |
| LSA1-TER | NSRDSKEISIIEKTNRESITTNVEGRRDIHKGHKGHL | |
| LSA3 (M23) | LSA3-NRI | DELFNELLNSVDVNGEVKENILEESQ |
| LSA3-NRII/Lipo | LEESQVNDDIFNSLVKSVQQEQQHNV K(Pam)-NH2 | |
| LSA3-RE | VESVAPSVEESVAPSVEESVAENVEESV | |
| LSA3-CT1/Lipo | LLSNIEEPKENIIDNLLNNI K(Pam)-NH2 | |
| SALSA (M4) | SALSA-1/Lipo | SAEKKDEKEASEQGEESHKKENSQESA K(Pam)-NH2 |
| SALSA-2 | NGKDDVKEEKKTNEKKDDGKTDKVQEKVLEKSPK | |
| STARP (M48) | STARP-R/Lipo | STDNNNTKTISTDNNNTKTI K(Pam)-NH2 |
| STARP-M/Mixotope/Lipo | STDNNNTKTISTDNNNTNTI K(Pam)-NH2 | |
| L---T-NTIKA---S-IT-N | ||
| ----D--D-NL------D-T | ||
| -------K---------K-K | ||
| Adjuvant (V63) | Montanide ISA-51 |
a
Aotus monkeys were immunized with combinations of (i) lipopeptides (Lipo) injected in phosphate-buffered saline without an adjuvant at one location (pooled when there was more than one lipopeptide from each molecule) and (ii) pools of peptides emulsified with Montanide ISA-51 adjuvant (SEPPIC, Paris, France) injected on the same day at another site. STARP-M/Mixotope/Lipo consists of a mixed-epitope degenerated sequence as indicated above and described in reference 20.
b
Peptide linked to palmitic acid (Pam) via a lysine residue.