TABLE 2.
Monkey | Antigen | ELISA titer on post- immunization day:
|
|
---|---|---|---|
15 | 210 | ||
M21 | LSA1-J | <100b | <100 |
LSA1-NR | 2,700 | 8,100 | |
LSA1-REP | 100 | 300 | |
LSA1-TER | 2,700 | 8,100 | |
M23 | LSA3-NRI | <100 | <100 |
LSA3-NRII | 100 | 100 | |
LSA3-RE | 100 | 100 | |
LSA3-CT1 | 100 | 100 | |
M4 | SALSA-1 | 100 | 100 |
SALSA-2 | 2,700 | 8,100 | |
M48 | STARP-R | 2,700 | 2,700 |
STARP-M | 24,300 | 8,100 |
Titers determined in samples taken 15 and 210 days postimmunization correspond to the dilution of the test sera whose optical density at 450 nm was above the mean of control Aotus sera plus 2 standard deviations. Controls included (i) sera from 10 Aotus monkeys with no history of exposure to malaria, as well as preimmunization sera from the immunized monkeys, and (ii) peptides RESA and MSP3. The mean optical densities of the control Aotus sera against each of the peptides, used to define the cutoff values, ranged between 0.02 and 0.18.
Negative at a dilution of 1:100.