TABLE 3.

IFAT determination of stage-specific antibody responses^a

Monkey	Source of immunizing peptides	Sporozoites		Liver stage parasites
		Day 15	Day 210	Day 15	Day 210
M21	LSA1	<20b	<20	100	100
M23	LSA3	100	100	100	100
M4	SALSA	100	100	<20	<20
M48	STARP	3,200	3,200	200	100

^aTwofold serial dilutions in phosphate-buffered saline of serum from each monkey were tested in an IFAT, starting at 1:20. Each value is the reciprocal of the highest positive antibody dilution. Rabbit anti-Saimiri monkey IgG diluted 1/200 was employed, as in an ELISA, as the second antibody and detected with fluorescein-conjugated goat anti-rabbit IgG (heavy and light chains; Diagnostic Pasteur France) diluted at 1/100. Sera from 10 Aotus monkeys with no history of malaria were used as controls and found to be negative at a dilution of 1:20. 

^bNegative at a dilution of 1:20.