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. 1998 Jul;66(7):3423–3428. doi: 10.1128/iai.66.7.3423-3428.1998

TABLE 3.

IFAT determination of stage-specific antibody responsesa

Monkey Source of immunizing peptides Sporozoites
Liver stage parasites
Day 15 Day 210 Day 15 Day 210
M21 LSA1 <20b <20 100 100
M23 LSA3 100 100 100 100
M4 SALSA 100 100 <20 <20
M48 STARP 3,200 3,200 200 100
a

Twofold serial dilutions in phosphate-buffered saline of serum from each monkey were tested in an IFAT, starting at 1:20. Each value is the reciprocal of the highest positive antibody dilution. Rabbit anti-Saimiri monkey IgG diluted 1/200 was employed, as in an ELISA, as the second antibody and detected with fluorescein-conjugated goat anti-rabbit IgG (heavy and light chains; Diagnostic Pasteur France) diluted at 1/100. Sera from 10 Aotus monkeys with no history of malaria were used as controls and found to be negative at a dilution of 1:20. 

b

Negative at a dilution of 1:20.