FIG. 3.
RT-PCR of M. gallisepticum RNA. (A) Agarose gel of RT-PCR results. (B) Gel transferred to a nylon membrane and hybridized with a 32P-labeled oligonucleotide from within mgc2. Lanes 1, M. gallisepticum tuf gene; lanes 2, mgc1; lanes 3, mgc2; lanes 4, RNase-treated mgc2; lanes 5, RNA template without reverse transcriptase. The photographed gel and nylon membrane blot were scanned on an Apple Color I scanner, cropped with Photoshop (Adobe Systems Inc., Mountain View, Calif.), and enlarged and labeled with QuarkXPress (Quark, Inc., Denver, Colo.).