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| Gene | Name | Primer sequence | Annealing temperature(°C) | Amplicon size(bp) |
|---|---|---|---|---|
| gltA | F | GTCGGTTCTCTTTCAGCATT | 57 | 311 |
| R | CCGGCAATTCTTACTGTTGA | 57 | ||
| ompB | F | GCAACAACTACAGGAACCAC | 56 | 254 |
| R | AACTGGCTACTTCCGATAGC | 56 | ||
| 16S rRNA | F | TGATCCAGCAATACCGAGT | 56 | 382 |
| R | TGATCCAGCAATACCGAGT | 55 | ||
| groEL | F | AGGTCCAAAAGGAAGAAACG | 56 | 354 |
| R | GTACCGACCTGTGCTATTTC | 56 | ||
| sca4 | F | CACTGCTCACTACGAAGAAG | 56 | 358 |
| R | TGAAGATTCAGCTTGTTGCA | 56 |
