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. 2024 Feb 2;10(5):eadj3808. doi: 10.1126/sciadv.adj3808

Fig. 2. GPR39 was integral to normal glycinergic input onto SOM+ interneurons.

Fig. 2.

(A) GlyR-mIPSCs recorded on SOM+ interneurons at day 21 after intraspinal injection of AAV2/9-EF1α-DIO-EGFP (EGFP) or AAV2/9-EF1α-DIO-shRNA-GPR39-EGFP (shRNA) in SOM-Cre mice. ***P < 0.001 and ns, P = 0.605 (Mann-Whitney U test). n = 9 neurons from six mice per group. (B) Immunofluorescence for GPR39 and gephyrin at day 21 after intraspinal injection of AAV2/9-EF1α-DIO-EGFP in SOM-Cre mice. The boxed area (top) was shown with higher magnification (bottom). The arrow indicated the colocalization. Scale bars, 5 μm (top) and 2 μm (bottom). (C) Current-clamp recordings of AP firings elicited by depolarizing current injections in SOM+ interneurons expressing EGFP or shRNA (left). The firing frequencies at all current injection levels were quantified (right). F23,874 = 6.909, ***P < 0.001 (repeated measurement). n = 20 neurons from 10 mice per group. (D to F) Rheobase (D, ***P < 0.001, Mann-Whitney U test, n = 12 neurons per group), membrane resistance (E, *P = 0.014, Mann-Whitney U test, n = 9 neurons per group), and resting membrane potential (F; **P = 0.002, Mann-Whitney U test, n = 11 neurons per group) of SOM+ interneurons expressing EGFP or shRNA. (G) Current-clamp recordings of AP firings evoked by electrical stimulation of dorsal roots at β-amyloid (Aβ) fiber intensity (25 μA) on SOM+ neurons expressing EGFP or shRNA before and after strychnine (2 μM) perfusion. **P = 0.003 (χ2 = 8.925) and ##P = 0.009 (χ2 = 6.832), Pearson’s chi-square test. In, inhibitory neurons.