KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Bacterial and Virus Strains | ||
| E. coli: Strain OP50 | Caenorhabditis Genetics Center (CGC) | OP50 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Rhodamine 110 | Millipore Sigma |
Cat#83695 |
| Rhodamine B | Millipore Sigma |
Cat#83689 |
| Deposited Data | ||
| Original code and data related to recording and analyzing neural activity and behavior | This paper | Data: https://doi.org/10.5281/zenodo.8150515 and www.wormwideweb.org Code: https://doi.org/10.5281/zenodo.8151918 and https://github.com/flavell-lab/AtanasKim-Cell2023 |
| Experimental Models: Organisms/Strains | ||
| C. elegans: flvIsl 7[tag-168: :NLS-GCaMP7F, gcy-28.d::NLS-tag-RFPt, ceh-36:NLS-tag-RFPt, inx-1::tag-RFPt, mod-1 ::tag-RFPt, tph-1 (short)::NLS-tag-RFPt, gcy-5::NLS-tag-RFPt, gcy-7::NLS-tag-RFPt]; flvIs18[tag-168: :NLS-mNeptune2.5]; lite-1(ce314); gur-3(ok2245) | This paper | SWF415 |
| C. elegans: flvIs17; otIs670 [low-brightness NeuroPAL]; lite-1(ce314); gur-3(ok2245) | This paper | SWF702 |
| C. elegans: flvEx450[eat-4::NLS-GFP, tag-168: :NLS-mNeptune2.5]; lite-1(ce314); gur-3(ok2245) | This paper | SWF360 |
| C. elegans: flvEx451[tag-168::NLS-GFP, tag-168::NLS-mNeptune2.5]; lite-1(ce314); gur-3(ok2245) | This paper | SWF467 |
| C. elegans: flvEx207[nlp-70::HisCl1, elt-2::nGFP] | This paper | SWF515 |
| C. elegans: flvEx301[tbh-1:: TeTx: :sl2-mCherry, elt-2::nGFP] | This paper | SWF688 |
| C. elegans: flvEx481[flp-8::inv[unc-103-sl2-GFP], ceh-6::cre, myo-2::mChrimson] | This paper | SWF996 |
| C. elegans: flvEx482[unc-25::inv[unc-103-sl2-GFP], flp-22::cre, myo-2::mChrimson] | This paper | SWF997 |
| C. elegans: kyEx4268 [mod-1::nCre, myo-2::mCherry]; kyEx4499 [odr-2(2b)::inv[TeTx::sl2GFP], myo-3::mCherry] | This paper | SWF703 |
| C. elegans: leIs4207 [Plad-2::CED-3 (p15), Punc-42::CED-3 (p17), Plad-2::GFP, Pmyo-2::mCherry] | This paper | UL4207 |
| C. elegans: leIs4230 [Pflp-12s::CED-3 (p15), Pflp-12s::CED-3 (p17), Pflp-12s::GFP, Pmyo-2::mCherry] | This paper | UL4230 |
| C. elegans: flvEx485[gcy-21::Chrimson-t2a-mScarlett, elt-2::nGFP] | This paper | SWF1000 |
| C. elegans: flvEx502[ceh-28::GtACR2-t2a-GFP, myo-2::mCherry] | This paper | SWF1026 |
| C. elegans: flvEx499[ceh-19::inv[GtACR2-sl2-GFP], ins-10::nCre, myo-2::mCherry] | This paper | SWF1023 |
| Recombinant DNA | ||
| pSF300 [tag-168 ::NLS-GCaMP7F] | This paper | pSF300 |
| pSF301[tag-168::NLS-mNeptune2.5] | This paper | pSF301 |
| pSF302[tag-168::NLS-GFP] | This paper | pSF302 |
| pSF303 [tag-168::NLS-tag-RFPt] | This paper | pSF303 |
| Software and Algorithms | ||
| NIS-Elements (v4.51.01) | Nikon | https://www.nikoninstruments.com/products/software |
| Other | ||
| Zyla 4.2 Plus sCMOS camera | Andor | N/A |
| Ti-E Inverted Microscope | Nikon | N/A |