Figure 5.

Enhanced SRRM4 expression confers more proliferative phenotype to breast cancer cells: (A) relative BrdU incorporation (luminescence) in SKBR3 control scramble, SRRM4^OE, SRRM4^KD cells (n = 2 per sample, 3 wells per experiment). (B) Relative mRNA expression of dormancy markers p53, SOX9, RARB, TGFB3 in SKBR3, SRRM4^OE, SRRM4^KD cells acclimated to nutrient-deficient media (mean and SEM, n = 3 per sample). (C) Representative IF images and comparison of nuclear colocalization of SOX9 in SKBR3 scramble SRRM4^OE, SRRM4^KD cells in control (CC) and nutrient-deficient (NDC) conditions, respectively. Data presented as percent nuclear colocalization per cell in both conditions. (D–F) In vitro competitive colonization model. Representative IF images and quantification of total cell numbers for each tumor cell type in 3 different combination cocultures with primary neurons: (D) SKBR3 (GFP)/SRRM4^OE (mCherry), (E) SRRM4^OE (mCherry)/SRRM4^KD (GFP), (F) SKBR3 (mCherry)/SRRM4^KD (GFP). Individual values in graph represent number of cells of each type (mean and SEM) per image quantified (>6 images/coculture sample/timepoint).