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. 2023 Sep 16;26(2):309–322. doi: 10.1093/neuonc/noad175

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Figure 6. — SRRM4 facilitates CNS acclimation in breast cancer cells in the neuronal microenvironment: (A) SRRM4 and REST4 expression in SKBR3^ctrl cells in neuron-conditioned media (NCM). Data represented as fold change in expression relative to untreated SKBR3 cells. (B) Comparison of nuclear SRRM4 and REST4 in SRRM4^OE cells in 2 conditions (tumor only, tumor–neuron coculture). Data represented in graph as percent nuclear colocalization per cell for both conditions (3 images per sample, 4 tumor cells per image). (C) Relative expression of SRRM4 and REST4 in SRRM4^OE and SRRM4^KD cells in 2 conditions (cells only, cells treated with NCM for 48 hours). Red bars (plain, striped) represent SRRM4^OE cells; blue bars (plain, striped) represent SRRM4^KD cells (3 wells/sample/condition). (D) Clustergram representing differential mRNA expression of CNS-specific (neurotransmitter receptors and synaptic plasticity mediators) in SKBR3 cells (control, SRRM4^OE, and SRRM4^KD) in 2 conditions (tumor cells only, tumor cells grown in neuron-conditioned media). qPCR target validation for relative expression of SRRM4-dependent target genes (E) CNR1, (F) BDNF, (G) RAB3A, (H) GRIN2B, (I) TACR3, (J) RELN, and (K) GRM8 in tumor cells in 2 conditions (tumor only, tumor cells treated with NCM, 3 wells/sample/condition).