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. 2023 Dec 14;43(6):406–419. doi: 10.1038/s41388-023-02914-0

Fig. 1. PIM1 kinase is essential for LD accumulation in prostate cancer.

Fig. 1

A Representative images of LDs in PC3TripzPIM1 cells following doxycycline treatment (50 ng/ml, 24 h). B Western blot of PC3TripzPIM1 cells following doxycycline treatment (50 ng/ml, 24 h). C Quantification of LD size and number per nuclei (n > 30 cells/group). D Representative images of basal levels of LDs, and (E) western blotting for PIM1 expression in the indicated cell lines. F Quantification of LD size and number per nuclei (n > 30 cells/group). G Representative images of LDs in tumor sections from PC3 and PC3-PIM1 xenograft tumors; LDs are in red (Oil Red O) and nuclei in blue (Hematoxylin). H Quantification of oil red o staining as % stained cells. At least 30 cells from 6 random fields were analyzed per treatment group. n = 3, mean ± SEM. ***p ≤ 0.001. For staining in (A) and (D), LDs in green (LipidSpot488) and nuclei in blue (Dapi). Scale bars, 50 µm. n = 3, mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.